BIO PRODUCT
GenCro™ PCR consumables
GenCro™ Purification columns
BIO PRODUCTGenCro™ Purification columns

GenCro™ Purification columns





HYUNDAI MICRO supplies spin columns to numerous domestic and overseas manufacturers of nucleic acid isolation and purification kit. Depending on the characteristics of the kits, there are various types of columns that can obtain high-yield and high-purity nucleic acid.
HM proposes optimal samples to achieve maximum extraction efficiency based on each manufacturer’s reagent composition. And can choose the best matching product through the testing. GenCro Spin column adopts the high quality silica membrane from a famous filter manufacturer and HM directly manages the production of the filter material to ensure the best efficiency.
In addition, use the high-quality plastic materials and produces them in automated facilities under the ISO 9001:2015 Quality Assurance System to maintain the stability of quality.



GenCro™ Spin Columns

  • Various composition of filter media Depending on the reagents of the kit manufacturer, HM suggests the best composition of filter layers to achieve maximum extraction efficiency.
    GenCro Spin column adopts the high quality silica membrane from a famous manufacturer and directly manages the production of the filter material to ensure the stability of quality.
  • Customized spin column HM has various options for customized column, from the color of the tube to the shape of the O-ring and they are choosable. Clear, Blue, Yellow, Purple in tube color options and Flat pattern, Sawtooth pattern in O-ring types. (Sawtooth O-ring is excellent at removing ethanol in the washing.)
  • Compatible with remaining buffers Through the testing of provided free samples, you can find and select the column that is compatible with the remaining buffers in your current kit. There are no buffers that are thrown away, saving money.
  • Sterilization / OEM production Seterilization and OEM production can be responded upon the request from the customer. OEM manufacturing is progressed in automated facilities under ISO 9001:2015 Quality Assurance System, and is more flexible in responding to quantity and packaging types.
    High quality electron beam sterilization is provided and certificate is issued.


Mini Spin Column

  • ▲ H4211G

  • ▲ H4201G (Without Cap)

GenCro™ mini-spin column is compatible with a wide range of buffers from famous brands.
HYUNDAI MICRO has various formats of spin column that can cover from DNA to RNA. Available two types of tubes, with lid and without lid. Clear, blue, yellow, and purple are in four colors. Two types of O-ring, Flat and Sawtooth pattern are choosable depending oncharacteristics of reagent. Suitable for both Centrifuge and Vacuum method.
Supplied with a 2ml collection tube, and the columns for RNA extraction are individually packed and sterilized.

> RNase-/DNase-free
> Nonpyrogenic
> Nonsterile/Sterile(for RNA)
> Silica membrane

  • ▲ H5111G (Capsule type / Sterile)

  • ▲ H4002A (Adaptor for 2ml column)



Pre-Filtration Column

When extracting the Total RNA, use before the Capsule type column(sterile).
During the Lysis process, removes the cell debris to prevent column blockage for improving the final RNA extraction efficiency. Only used for pre-filtration purposes, it doesn't have DNA /RNA binding capacity.

> RNase-/DNase-free
> Nonpyrogenic
> Nonsterile
> PE membrane filter

▲ H6211G(Pre-filtraton)



Product Cat no. Volume Color Filter Composition Packing
Spin column H4211GC 2ml(0.8ml) Clear Silica Spin column, Collection tube 50ea/p.k
Spin column(without cap) H4201GC 2ml(0.8ml) clear Silica Spin column, Collection tube 50ea/p.k
Spin column(Bulk) H4211GCK 2ml(0.8ml) Clear Silica Spin column, Collection tube 500ea/p.k
Spin column(Sterilized) H5111GC 1ml(0.8ml) Clear Silica Spin column, Collection tube 10ea/p.k
Spin column
(Pre-Filtration)		 H6211GC 2ml(0.8ml) Clear PE Spin column, Collection tube 50ea/p.k
Collection tube H4200T 2ml Clear - Collection tube 50ea/p.k
Spin column adaptor H4002A 20ml Clear - Spin column adapter
(for 2ml)		 5ea/p.k, 4p.k/box


Midi Column

Easy to isolate / purificate high-yield Plasmid DNA into a midi scale, ideal for both Centrifuge and Vacuum method.
Supplied 15 ml conical tube with midi column and conical tubes are available for most centrifuges. Use only column, it is compatible with most Vacuum Manifolds.

> RNase-/DNase-free
> Nonpyrogenic
> Nonsterile
> Silica membrane

▲ H4210DC(15ml)



Maxi Column

Easy to isolate / purificate high-yield Plasmid DNA into a maxi scale, ideal for both Centrifuge and Vacuum method. Supplied 50 ml conical tube with midi column and conical tubes are available for most centrifuges. Use only column, it is compatible with most Vacuum Manifolds.

> RNase-/DNase-free
> Nonpyrogenic
> Nonsterile
> Silica membrane

▲ H4210AC(50ml)



Product Cat no. Volume Color Filter Composition Packing
Midi column H4210DC 15ml Clear Silica 15ml tube + Midi column 5ea/p.k, 4p.k/box
Maxi column H4210AC 50ml Clear Silica 50ml tube + Midi column 4ea/p.k, 5p.k/box


96-well binding plate

Up to 96 samples can be easily isolated and purified at the same time.
Use high-quality Silica membrane, ensure the high-yield and stability. (Collection plate is not included.)

> RNase-/DNase-free
> Nonpyrogenic
> Nonsterile
> Silica membrane

▲ H4201WC



Product Cat no. Volume Color Filter Composition Packing
96-well binding plate H4201WC 1.5ml/well Clear Silica 96-well binding plate 1ea/p.k, 6p.k/box


Product selection guide

HM offers a wide range of options for manufacturers of nucleic acid purification kit. Optimal column for your reagent can be selected by the following list.

Step 1. Column Types

Product type Standard Color
Column with cap 2ml(0.8ml) Natural
Violet
Blue
Yellow
Column with out cap 2ml(0.8ml)
Medi column 15ml
Maxi column 50ml
96-well plate 1.5ml/well
※ You can choose the color.

Step 2. O-Ring

O-Ring type color
Sawtooth-pattern Natural
Green
Blue
Red
Violet
Flat-pattern

Step 3. Filter Material

Filter layer Application
Samples will be
provided for the
testing for each
application.

HM will provide the
different filter latered
spin columns for the
maximum efficiency
of manufacturer's
reagent composition. 		Plasmid prep kit
Genomic prep kit
PCR Purification kit
Gel Purification kit
Total RNA prep kit
Plant DNA prep kit
Blood RNA prep kit
Bacteria DNA prep kit
Viral Nucleic acid prep kit

Step 4. Package

  • Select Options
  • Package material
  • Package design
  • Standard packing unit
    (Individual, Bulk, Small quantity packing)
  • Sterilization
  • ▼
  • Final decision
※ The choice can be limited under few conditions.


Compatible test_Supplier “Q” and GenCro™ spin column.

1. Genomic DNA Extraction

1-1. Mammalian cell culture< Sample : HeLa cell (1x106 cell) >

  • Yield(㎍)
  • 8.99
  • 7.87
  • 8.34
  • 7.23
  • 8.01
  • 7.72
  • 8.29
  • 7.83
  • A260/A280
  • 2.08
  • 2.05
  • 2.06
  • 2.03
  • 2.01
  • 2.00
  • 2.00
  • 2.00

1-2. Blood< Sample : Human blood (100µℓ) >

  • Yield(㎍)
  • 1.82
  • 1.98
  • 1.79
  • 2.23
  • 2.26
  • 1.87
  • 1.75
  • 1.76
  • A260/A280
  • 1.88
  • 1.99
  • 1.86
  • 1.89
  • 1.89
  • 1.94
  • 1.89
  • 1.89

1-3. Plant< Sample : Brassica oleracea (100mg) >

  • Yield(㎍)
  • 5.03
  • 4.65
  • 5.77
  • 4.40
  • 6.33
  • 6.38
  • 5.74
  • 5.30
  • A260/A280
  • 1.88
  • 1.08
  • 1.81
  • 1.78
  • 1.80
  • 1.81
  • 1.82
  • 1.84

1-4. Animal Tissue< Sample : Mouse kidney (20mg) >

  • Yield(㎍)
  • 18.38
  • 21.77
  • 25.76
  • 21.80
  • 14.62
  • 15.98
  • 13.64
  • 16.53
  • A260/A280
  • 1.92
  • 1.92
  • 1.90
  • 1.93
  • 1.94
  • 1.94
  • 1.95
  • 1.92

1-5. Bacterium< Sample : E. coli (5x108 cell) >

  • Yield(㎍)
  • 4.6
  • 4.12
  • 4.14
  • 4.62
  • 3.62
  • 4.32
  • 4.06
  • 4.2
  • A260/A280
  • 2.03
  • 1.97
  • 1.93
  • 1.94
  • 1.96
  • 1.92
  • 1.96
  • 1.94


2. Plasmid DNA Extraction & Fragment DNA Purification

2-1. High-copy plasmid< Sample : Puc19 plasmid, 2ml culture >

  • Yield(㎍)
  • 1.63
  • 1.71
  • 1.32
  • 1.11
  • 1.74
  • 1.91
  • 2.06
  • 1.99
  • A260/A280
  • 1.90
  • 1.89
  • 1.88
  • 1.78
  • 1.89
  • 1.84
  • 1.89
  • 1.90

2-2. Low-copy plasmid< Sample : Pet28b plasmid, 2ml culture >

  • Yield(㎍)
  • 1.21
  • 1.14
  • 1.45
  • 1.27
  • 1.05
  • 0.78
  • 1.50
  • 1.30
  • A260/A280
  • 1.92
  • 1.82
  • 1.88
  • 1.88
  • 1.86
  • 1.85
  • 1.85
  • 1.90

2-3. PCR product< Sample : 500bp PCR product >

  • Yield(㎍)
  • 1.50
  • 1.53
  • 1.47
  • 1.49
  • 1.64
  • 1.57
  • 1.46
  • 1.41
  • A260/A280
  • 1.88
  • 1.98
  • 1.97
  • 1.95
  • 1.91
  • 1.88
  • 1.91
  • 1.89

2-4. Gel purification< Sample : 500bp PCR product >

  • Yield(㎍)
  • 1.07
  • 1.13
  • 1.15
  • 1.11
  • 1.12
  • 1.20
  • 1.13
  • 1.13
  • A260/A280
  • 1.92
  • 1.97
  • 1.94
  • 1.90
  • 1.86
  • 2.28
  • 1.91
  • 1.97


3. RNA Extraction

3-1. Mammalian cell culture< Sample : HeLa cell (1x106 cell) >

  • Yield(㎍)
  • 15.40
  • 16.23
  • 19.69
  • 17.21
  • 18.01
  • 17.61
  • 15.33
  • 18.55
  • A260/A280
  • 2.06
  • 2.06
  • 2.06
  • 2.07
  • 2.06
  • 2.07
  • 2.07
  • 2.05

3-2. Plant< Sample : Brassica oleracea (100mg) >

  • Yield(㎍)
  • 40.35
  • 39.55
  • 45.69
  • 66.28
  • 33.77
  • 34.02
  • 58.09
  • 43.08
  • A260/A280
  • 2.06
  • 2.08
  • 2.05
  • 2.07
  • 2.06
  • 2.07
  • 2.07
  • 2.05

3-3. Bacterium< Sample : E. coli (1x108 cell) >

  • Yield(㎍)
  • 4.42
  • 4.50
  • 4.85
  • 5.05
  • 5.47
  • 5.88
  • 5.04
  • 3.62
  • A260/A280
  • 2.06
  • 2.08
  • 2.05
  • 2.07
  • 2.06
  • 2.07
  • 2.07
  • 2.05

3-4. Animal Tissue< Sample : Mouse kidney (20mg) >

  • Yield(㎍)
  • 52.39
  • 31.09
  • 58.91
  • 36.50
  • 46.51
  • 43.32
  • 49.20
  • 46.00
  • A260/A280
  • 2.06
  • 2.08
  • 2.05
  • 2.07
  • 2.06
  • 2.07
  • 2.07
  • 2.05